Product Introduction
L-Alanine is an organic compound with the molecular formula C3H7NO2, appearing as a colorless to white crystalline powder. It is soluble in water and ethanol, but insoluble in ether and acetone. It is primarily used in biochemical research, tissue culture, liver function testing, as a flavor enhancer, to improve the taste of seasonings, and as an acid taste corrector to modify the acidity of organic acids.
L-Alanine Production Process
Chemical Synthesis Method:
- Propionic acid chlorination and amination method: Propionic acid is used as raw material, chlorinated with liquid chlorine at 105°C in the presence of 3% red phosphorus catalyst to produce 2-chloropropionic acid, which is then introduced into ammonia water with hexamethylenetetramine as a catalyst at 60°C for amination to generate 2-aminopropionic acid. The reaction mixture is then crystallized in methanol, centrifuged, and dried to obtain the final product, α-alanine.
- α-Bromopropionic acid chlorination method: α-Bromopropionic acid, ammonia water, and ammonium bicarbonate are mixed, stirred, and refluxed for 7 hours, then evaporated to dryness. The residue is washed with ethanol to remove ammonium bromide, crystallized, decolorized, filtered, and recrystallized with ethanol to obtain the final product.
- Cyanohydrin method: Acetaldehyde reacts with hydrogen cyanide to form cyanohydrin, which then reacts with ammonia to obtain aminonitrile. Hydrolysis under alkaline conditions yields sodium aminopropionate, which is then ion-exchanged to obtain L-alanine.
Enzymatic Method:
Immobilized Enzyme Method
Acrylonitrile Method: Acrylonitrile reacts with ammonia in a diphenylamine and tert-butanol solution at 109°C and 1176.798KPa pressure to produce β-aminopropionitrile, which then reacts with sodium hydroxide to generate sodium β-aminopropionate. Acidification with hydrochloric acid finally yields β-aminopropionic acid.
β-Aminopropionitrile Method: β-Aminopropionitrile is hydrolyzed and acid-separated to obtain the final product.
Tobacco: BU, 21; FC, 20. Experiments using acid hydrolysis of silk fibers (degelatinized white silk) for preparation.
Obtained orthorhombic crystals from water.
Fermentation Extraction Process:
The fermentation process for L-alanine extraction is illustrated. The fermentation broth undergoes ultrafiltration to remove cells and large molecular proteins, followed by pH adjustment and passage through 732-type strong acid cation exchange resin (H+ type). L-Alanine is exchanged onto the resin, while most pigments, soluble proteins, and anions are washed away with the overflow wastewater. The resin-bound L-Alanine is fully eluted with 4% ammonia, and the product is obtained through vacuum ammonia removal, activated carbon decolorization, concentration, crystallization, centrifugation, and drying. The purified mother liquor from centrifugation is recycled to improve extraction yield.
L-Alanine Benefits and Functions
Omitted
L-Alanine Application Scenarios
Pharmaceuticals:
L-Alanine is an important raw material for the synthesis of VB6 and is a component of the nutritional supplement "Amino Acid Nutritional Infusion."
Food Additives:
(1) Enhances the nutritional value of foods in various types of foods and beverages, such as bread, ice cream, fruit tea, dairy products, carbonated beverages, and more. Adding 0.1-1% alanine can significantly improve protein utilization in foods and beverages. Due to alanine’s ability to be directly absorbed by cells, it can quickly restore energy and refresh the body after consumption.
(2) Improves the taste of artificial sweeteners, enhances sweetness, and reduces the amount used. Adding 1-10% alanine to compound sweeteners can increase sweetness, making it as smooth as natural sweeteners and improving flavor. Alanine is also a key raw material for the synthesis of high-sweetness alitame (Alitame, L-aspartyl-D-alaninamide, 600 times sweeter than sucrose).
Biochemical Research, Tissue Culture, Liver Function Testing.
Used as a flavor enhancer. Enhances the taste of seasonings and can also be used as an acid taste corrector to improve the acidity of organic acids.
β-Alanine is used as an additive in chemical plating and electroplating and for the preparation of electroplating corrosion inhibitors.
L-Alanine Packaging and Storage
Storage Conditions: This product should be sealed and kept away from light, stored in a cool, dry, and well-ventilated place.
Packaging: Bulk: 25kg/cardboard drum, Small sample: 1kg/aluminum foil bag, can also be packaged according to customer requirements.
Shipping Method: Express or logistics, domestic express delivery within three days, logistics within five days. Prices generally include domestic shipping costs.
Shelf Life: Two years
